Sulfadiazine biodegradation by Phanerochaete chrysosporium: Mechanism and degradation product identification

Zhang，Ting1,2; Cai，Ling3; Xu，Bentuo4; Li，Xicheng1,2; Qiu，Wenhui1,2; Fu，Caixia1,2; Zheng，Chunmiao1,2

2019-12-01

10.1016/j.chemosphere.2019.124418

CHEMOSPHERE   影响因子和分区

0045-6535

1879-1298

Antibiotic contaminants have become a severe environmental problem in recent years and finding effective ways to deal with this issue is of great importance. In this study, Phanerochaete chrysosporium was used to degrade sulfadiazine (SDZ), which is frequently detected in the culture medium of isolates from soil and surface water systems. The results demonstrate that 10 mg L SDZ can be completely degraded by P. chrysosporium under conditions of pH 5.7 and 30 °C within 6 days. The Q-Exactive-MS/MS analysis identified and confirmed several different SDZ degradation intermediates, and four proposed degradation pathways of SDZ were deduced. Moreover, enzyme activity tests revealed that manganese peroxidase and ligninolytic peroxidase played important roles in SDZ degradation. Moreover, a transcriptome analysis method was performed to explore the mechanism and pathways of SDZ degradation by P. chrysosporium in greater detail. The results of GO and KEGG analysis strongly suggest that the metabolism pathway is significantly activated and plays an important role in antibiotic degradation. Further, this is the first study to identify SDZ degradation intermediates and two main intermediates were found to be involved in possible SDZ degradation pathways. This study is also the first report results from RNA sequencing to evaluate genome-wide changes of P. chrysosporium to further explore SDZ degradation mechanism.

Antibiotics Biodegradation RNA sequencing Sulfadiazine Transcription analysis

SCI ; EI

英语

第一 ; 通讯

Southern University of Science and Technology[] ; [2016YFC0402806] ; Natural Science Foundation of Fujian Province[2018J05066] ; Shenzhen Science and Technology Innovation Commission[JCYJ20180302180205159] ; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology[2017B030301012] ; National Natural Science Foundation of China[21607091]

Environmental Sciences & Ecology

Environmental Sciences

WOS:000496896700073

Elsevier Ltd

20193107253285

Antibiotics ; Degradation ; Enzyme activity ; RNA ; Surface waters

Surface Water:444.1 ; Bioengineering and Biology:461 ; Chemical Reactions:802.2

ENVIRONMENT/ECOLOGY

2-s2.0-85069873053

Scopus

1.Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution ControlSchool of Environmental Science and EngineeringSouthern University of Science and Technology,Shenzhen,518055,China
2.State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution ControlSchool of Environmental Science and EngineeringSouthern University of Science and Technology,Shenzhen,518055,China
3.Third Institute of OceanographyMinistry of Natural ResourcesPR China,Xiamen,361005,China
4.College of Life and Environmental ScienceWenzhou University,Wenzhou,325035,China

Zhang，Ting,Cai，Ling,Xu，Bentuo,et al. Sulfadiazine biodegradation by Phanerochaete chrysosporium: Mechanism and degradation product identification[J]. CHEMOSPHERE,2019,237.

Zhang，Ting.,Cai，Ling.,Xu，Bentuo.,Li，Xicheng.,Qiu，Wenhui.,...&Zheng，Chunmiao.(2019).Sulfadiazine biodegradation by Phanerochaete chrysosporium: Mechanism and degradation product identification.CHEMOSPHERE,237.

Zhang，Ting,et al."Sulfadiazine biodegradation by Phanerochaete chrysosporium: Mechanism and degradation product identification".CHEMOSPHERE 237(2019).