Long-Term In Vivo Glucose Monitoring by Polymer-Dot Transducer in an Injectable Hydrogel Implant

Liu, Jing1,2; Fang, Xiaofeng1; Zhang, Zhe1; Liu, Zhihe1; Liu, Jie1; Sun, Kai1; Yuan, Zhen2; Yu, Jiangbo3; Chiu, Daniel T.3; Wu, Changfeng1

2022

10.1021/acs.analchem.1c04730

ANALYTICAL CHEMISTRY   影响因子和分区

0003-2700

1520-6882

Optical sensors have attracted a great deal of interest for glucose detection. However, their practical applications for continuous glucose monitoring are still constrained by operational reliability in subcutaneous tissues. Here, we show an implantable hydrogel platform embedded with luminescent polymer dots (Pdots) for sensitive and long-term glucose monitoring. We use Pdot transducer in a polyacrylamide hydrogel matrix to construct an implantable platform. The hydrogel-Pdot transducer showed bright luminescence with ratiometric response to glucose changes. The in vitro and in vivo sensitivities of the hydrogel implant were enhanced by varying the enzyme concentration and injection volume. After implantation, the hydrogel with Pdot transducer remained at the implanted site without migration for 1 month and can be removed from the subcutaneous tissue for further analysis. Our results indicate that the hydrogel-Pdot platform maintains the intrinsic sensing property with excellent stability during 1 month implantation, while fibrous capsule formation on the implant in some cases needs to be solved for long-term continuous glucose monitoring.

SCI ; EI

英语

NI期刊 ; NI论文

第一 ; 通讯

National Natural Science Foundation of China[81771930] ; National Key R&D Program of China[2020YFA0909000] ; Shenzhen Science and Technology Program["KQTD20170810111314625","JCYJ20210324115807021"] ; Shenzhen Bay Laboratory[SZBL2021080601002]

Chemistry

Chemistry, Analytical

WOS:000744945000001

AMER CHEMICAL SOC

20220511580824

Glucose ; Hydrogels ; Luminescence ; Polymeric implants ; Tissue

Biological Materials and Tissue Engineering:461.2 ; Prosthetics:462.4 ; Light/Optics:741.1 ; Colloid Chemistry:801.3 ; Chemical Products Generally:804 ; Organic Compounds:804.1

CHEMISTRY

Web of Science

1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China
2.Univ Macau, Fac Hlth Sci, Taipa 999078, Macao, Peoples R China
3.Univ Washington, Dept Chem & Bioengn, Seattle, WA 98195 USA

Liu, Jing,Fang, Xiaofeng,Zhang, Zhe,et al. Long-Term In Vivo Glucose Monitoring by Polymer-Dot Transducer in an Injectable Hydrogel Implant[J]. ANALYTICAL CHEMISTRY,2022,94(4):2195-2203.

Liu, Jing.,Fang, Xiaofeng.,Zhang, Zhe.,Liu, Zhihe.,Liu, Jie.,...&Wu, Changfeng.(2022).Long-Term In Vivo Glucose Monitoring by Polymer-Dot Transducer in an Injectable Hydrogel Implant.ANALYTICAL CHEMISTRY,94(4),2195-2203.

Liu, Jing,et al."Long-Term In Vivo Glucose Monitoring by Polymer-Dot Transducer in an Injectable Hydrogel Implant".ANALYTICAL CHEMISTRY 94.4(2022):2195-2203.