Construction of Dopamine-Releasing Gold Surfaces Mimicking Presynaptic Membrane by On-Chip Electrochemistry

Li, Jun1; Sun, Chun-Lin4; An, Pengrong1; Liu, Xiaoyan3; Don, Ruihua3; Sun, Jinghong1; Zhang, Xingyu1; Xie, Yanbo1; Qin, Chuanguang1; Zheng, Wenfu3; Zhang, Hao-Li4; Jiang, Xingyu2,3

2019-06-05

10.1021/jacs.9b01003

Journal of the American Chemical Society   影响因子和分区

0002-7863

We report a strategy to construct a dopamine releasing gold surface mimicking a presynaptic membrane on a microfluidic chip to simulate in vivo neural signaling. We constructed dopamine self-assembled monolayers (DA SAMs) by electrochemical deprotection of methyl group-protected DA SAMs on a gold surface. Electrochemically controllable release of DA SAMs can be realized by applying nonhydrolytic negative potential on the gold surface. Our method in constructing DA SAMs avoids the polymerization and protonation of DA molecules which may lead to the failure of the DA SAM formation. By combining microfluidics, we realized spatial and temporal controllable release of DA by electrochemistry from the gold surface. Furthermore, by culturing neurons on the patterned DA SAMs, the interface between the DA SAMs and the neurons could serve as a presynaptic membrane, and the spatiotemporal release of DA could modulate the neuron activity with high precision. Our study holds great promise in the fields of neurobiology research and drug screening.

SCI ; EI

英语

NI期刊 ; NI期刊 ; NI论文

通讯

Minister of Science and Technology of China[2017YFA0205901]

Chemistry

Chemistry, Multidisciplinary

WOS:000470939200029

AMER CHEMICAL SOC

20192407024275

Amines ; Electrochemistry ; Gold ; Gold metallurgy ; Microfluidics ; Neurons ; Self assembled monolayers ; Targeted drug delivery

Biology:461.9 ; Precious Metals:547.1 ; Microfluidics:632.5.1 ; Electrochemistry:801.4.1 ; Organic Compounds:804.1

Web of Science

1.Northwestern Polytech Univ, MOE Key Lab Space Appl Phys & Chem, Joint Lab Nanofluid & Interfaces LONI, Sch Nat & Appl Sci, Xian 710072, Shanxi, Peoples R China
2.Southern Univ Sci & Technol, Dept Biomed Engn, 1088 Xueyuan Rd, Shenzhen 518055, Guangdong, Peoples R China
3.Natl Ctr NanoSci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, CAS Ctr Excellence Nanosci, Beijing 100190, Peoples R China
4.Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ Chem SKLAOC, Lanzhou 730000, Gansu, Peoples R China

Li, Jun,Sun, Chun-Lin,An, Pengrong,et al. Construction of Dopamine-Releasing Gold Surfaces Mimicking Presynaptic Membrane by On-Chip Electrochemistry[J]. Journal of the American Chemical Society,2019,141(22):8816-8824.

Li, Jun.,Sun, Chun-Lin.,An, Pengrong.,Liu, Xiaoyan.,Don, Ruihua.,...&Jiang, Xingyu.(2019).Construction of Dopamine-Releasing Gold Surfaces Mimicking Presynaptic Membrane by On-Chip Electrochemistry.Journal of the American Chemical Society,141(22),8816-8824.

Li, Jun,et al."Construction of Dopamine-Releasing Gold Surfaces Mimicking Presynaptic Membrane by On-Chip Electrochemistry".Journal of the American Chemical Society 141.22(2019):8816-8824.