Microfluidics-based approaches for separation and analysis of circulating tumor cells

Yin, Jiaxiang1,2,4; Deng, Jinqi2,6; Du, Chang1,4,5; Zhang, Wei2; Jiang, Xingyu2,3,6

2019-08

10.1016/j.trac.2019.07.018

TRAC-TRENDS IN ANALYTICAL CHEMISTRY   影响因子和分区

0165-9936

1879-3142

Metastasis is the leading cause of epithelial cancer-associated mortality. The shedding of tumor cells into the circulatory system is a key step for metastasis. Circulating tumor cells (CTCs) are reliable biomarkers for cancer diagnostics and treatment. To obtain clinical information, accurate approaches for separating and analyzing CTCs are necessary. Microfluidic technologies have demonstrated their extraordinary abilities for separating and analyzing CTCs. In this paper, we present a comprehensive review of microfluidics-based approaches for CTCs separation and analysis. We first present the general methods for CTCs separation using the microfluidic chips, including physical properties-based approaches for CTCs enrichment and affinity-based approaches for CTCs capture, and compare the pros and cons of different separation technologies. Then we introduce microfluidics-based CTCs analysis on cellular and molecular levels and the biomedical applications of CTCs analysis. Finally, we summarize the development of microfluidics-based CTCs separation and analysis, and make a prospective of future research. (C) 2019 Elsevier B.V. All rights reserved.

Microfluidic CTCs Cell separation Cell analysis Biomedical applications

SCI ; EI

英语

通讯

Chinese Academy of Sciences[QYZDJ-SSW-SLH039]

Chemistry

Chemistry, Analytical

WOS:000482209800010

ELSEVIER SCI LTD

20193107259820

Cardiovascular system ; Diagnosis ; Diseases ; Medical applications ; Pathology ; Separation ; Tumors

Biological Materials and Tissue Engineering:461.2 ; Medicine and Pharmacology:461.6 ; Microfluidics:632.5.1 ; Chemical Operations:802.3

CHEMISTRY

Web of Science

1.South China Univ Technol, Sch Mat Sci & Engn, Dept Biomed Engn, Guangzhou 510641, Guangdong, Peoples R China
2.Natl Ctr NanoSci & Technol, CAS Ctr Excellence Nanosci, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing Engn Res Ctr BioNanotechnol, 11 Zhongguancun Beiyitiao, Beijing 100190, Peoples R China
3.Southern Univ Sci & Technol, Dept Biomed Engn, 1088 Xueyuan Rd, Shenzhen 518055, Guangdong, Peoples R China
4.South China Univ Technol, Natl Engn Res Ctr Tissue Restorat & Reconstruct, Guangzhou 510006, Guangdong, Peoples R China
5.Minist Educ, Key Lab Biomed Mat Sci & Engn, Guangzhou 510006, Guangdong, Peoples R China
6.Univ Chinese Acad Sci, 19 A Yuquan Rd, Beijing 100049, Peoples R China

Yin, Jiaxiang,Deng, Jinqi,Du, Chang,et al. Microfluidics-based approaches for separation and analysis of circulating tumor cells[J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY,2019,117:84-100.

Yin, Jiaxiang,Deng, Jinqi,Du, Chang,Zhang, Wei,&Jiang, Xingyu.(2019).Microfluidics-based approaches for separation and analysis of circulating tumor cells.TRAC-TRENDS IN ANALYTICAL CHEMISTRY,117,84-100.

Yin, Jiaxiang,et al."Microfluidics-based approaches for separation and analysis of circulating tumor cells".TRAC-TRENDS IN ANALYTICAL CHEMISTRY 117(2019):84-100.