On-Chip Multicolor Photoacoustic Imaging Flow Cytometry

Jin，Tian1; Zhang，Chen1; Liu，Fei1; Chen，Xingxing1; Liang，Guangru1; Ren，Fei2; Liang，Suzi4; Song，Chaolong3; Shi，Jianbing2; Qiu，Weibao4; Jiang，Xingyu1; Li，Kai1; Xi，Lei1

2021-06-15

10.1021/acs.analchem.0c05218

ANALYTICAL CHEMISTRY   影响因子和分区

0003-2700

1520-6882

On-chip imaging flow cytometry has been widely used in cancer biology, immunology, microbiology, and drug discovery. Pure optical imaging combined with flow cytometry to derive chemical, structural, and morphological features of cells provides systematic insights into biological processes. However, due to the high concentration and strong optical attenuation of red blood cells, preprocessing is necessary for optical flow cytometry while dealing with whole blood. In this study, we develop an on-chip photoacoustic imaging flow cytometry (PAIFC), which combines multicolor high-speed photoacoustic microscopy and microfluidics for cell imaging. The device employs a micro-optical scanner to achieve a miniaturized outer size of 30 × 17 × 24 mmand ultrafast cross-sectional imaging at a frame rate of 1758 Hz and provides lateral and axial resolutions of 2.2 and 33 μm, respectively. Using a multicolor strategy, PAIFC is able to differentiate cells labeled by external contrast agents, detect melanoma cells with an endogenous contrast in whole blood, and image melanoma cells in blood samples from tumor-bearing mice. The results suggest that PAIFC has sufficient sensitivity and specificity for future cell-on-chip applications.

SCI ; EI

英语

NI期刊 ; NI论文

第一 ; 通讯

WOS:000663935200005

20212610546931

Biochips ; Blood ; Dermatology ; Flow cytometry ; Laser recording ; Mammals ; Oncology ; Photoacoustic effect ; Photoacoustic microscopy ; Scanning

Bioengineering and Biology:461 ; Light/Optics:741.1 ; Optical Devices and Systems:741.3 ; Laser Applications:744.9 ; Imaging Techniques:746

CHEMISTRY

2-s2.0-85108325303

Scopus

1.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,518055,China
2.School of Materials Science and Engineering,Beijing Institute of Technology,Beijing,100081,China
3.School of Mechanical Engineering and Electronic Information,China University of Geosciences (Wuhan),Wuhan,430074,China
4.Research Center for Biomedical Imaging,Shenzhen Institutes of Advanced Technology,Shenzhen,518055,China

Jin，Tian,Zhang，Chen,Liu，Fei,et al. On-Chip Multicolor Photoacoustic Imaging Flow Cytometry[J]. ANALYTICAL CHEMISTRY,2021,93(23):8134-8142.

Jin，Tian.,Zhang，Chen.,Liu，Fei.,Chen，Xingxing.,Liang，Guangru.,...&Xi，Lei.(2021).On-Chip Multicolor Photoacoustic Imaging Flow Cytometry.ANALYTICAL CHEMISTRY,93(23),8134-8142.

Jin，Tian,et al."On-Chip Multicolor Photoacoustic Imaging Flow Cytometry".ANALYTICAL CHEMISTRY 93.23(2021):8134-8142.