Integrating a Concentration Gradient Generator and a Single-Cell Trapper Array for High-Throughput Screening the Bioeffects of Nanomaterials

Liu, Xiaoyan1,2,3,4; Jia, Yuexiao2; Han, Ziwei1; Hou, Qinghong2; Zhang, Wei1,4; Zheng, Wenfu1,4; Jiang, Xingyu1,2,4

2021-04-01

10.1002/anie.202101293

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION   影响因子和分区

1433-7851

1521-3773

We herein develop a concentration gradient generator (CGG) on a microfluidic chip for diluting different nanoparticles. Specifically designed compact disk (CD)-shaped microchannels in the CGG module could thoroughly mix the flowing solutions and generate a linear concentration gradient of nanoparticles without aggregation. We combine the CGG with a single-cell trapper array (SCA) on microfluidics to evaluate the concentration-dependent bioeffects of the nanoparticles. The precise control of the spatiotemporal generation of nanoparticle concentration on the CGG module and the single-cell-level monitoring of the cell behaviors on the SCA module by a high-content system in real time, render the CGG-SCA system a highly precise platform, which can exclude the average effect of cell population and reflect the response of individual cells to the gradient concentrations accurately. In addition, the CGG-SCA system provides an automated platform for high-throughput screening of nanomedicines with high precision and low sample consumption.

drug screening microfluidic chips nanoparticle dilution

SCI ; EI

英语

NI期刊 ; NI论文

通讯

National Key R&D Program of China["2018YFA0902600","2017YFA0205901"] ; National Natural Science Foundation of China[21535001,81730051,21761142006,81673039,32071390] ; High-level University Construction Fund from Shenzhen Government["G02386301","G02386401"] ; Guangdong Innovative and Entrepreneurial Research Team Program[2019ZT08Y191]

Chemistry

Chemistry, Multidisciplinary

WOS:000643795500001

WILEY-V C H VERLAG GMBH

20211810274102

Cell culture ; Cell proliferation ; Nanoparticles ; Screening

Biology:461.9 ; Microfluidics:632.5.1 ; Nanotechnology:761 ; Chemical Operations:802.3 ; Solid State Physics:933

CHEMISTRY

Web of Science

1.Natl Ctr NanoSci & Technol, CAS Ctr Excellence Nanosci, CAS Key Lab Biomed Effects Nanomat & Nanosafety, 11 Zhongguancun Beiyitiao, Beijing 100190, Peoples R China
2.Southern Univ Sci & Technol, Dept Biomed Engn, 1088 Xueyuan Rd, Shenzhen 518055, Guangdong, Peoples R China
3.Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China
4.Univ Chinese Acad Sci, 19 A Yuquan Rd, Beijing 100049, Peoples R China

Liu, Xiaoyan,Jia, Yuexiao,Han, Ziwei,et al. Integrating a Concentration Gradient Generator and a Single-Cell Trapper Array for High-Throughput Screening the Bioeffects of Nanomaterials[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2021,60:12319-12322.

Liu, Xiaoyan.,Jia, Yuexiao.,Han, Ziwei.,Hou, Qinghong.,Zhang, Wei.,...&Jiang, Xingyu.(2021).Integrating a Concentration Gradient Generator and a Single-Cell Trapper Array for High-Throughput Screening the Bioeffects of Nanomaterials.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,60,12319-12322.

Liu, Xiaoyan,et al."Integrating a Concentration Gradient Generator and a Single-Cell Trapper Array for High-Throughput Screening the Bioeffects of Nanomaterials".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 60(2021):12319-12322.