Reagents-loaded, automated assay that integrates recombinase-aided amplification and CaS12a nucleic acid detection for a point-of-care test

Chen，Yong1; Mei，Yixin1; Zhao，Xiaohui1; Jiang，Xingyu1,2

2020

10.1021/acs.analchem.0c03883

ANALYTICAL CHEMISTRY   影响因子和分区

0003-2700

1520-6882

Point-of-care testing for nucleic acid that combines amplification and readout is in great demand. This report integrates recombinase-aided amplification (RAA) with the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas12a system in a centrifugal microfluidic point-of-care test. We overcome the difficulty in the integration of these two processes, which mainly lies in the fact that Cas12a can digest the template DNA. We further integrate all reagents into the centrifugal microfluidics to automate the entire process. The Cas12a-assisted straightforward microfluidic equipment for analysis of nucleic acid (CASMEAN) enables us to rapidly and conveniently detect nucleic acid within 1.5 h. An application for detecting Pseudomonas aeruginosa confirms the excellent compatibility between RAA and the Cas12a system, which ensures the superior performance of CASMEAN, such as capabilities of point-of-care detection, high sensitivity, and high specificity. CASMEAN is a genetic detection platform with great potential.

SCI ; EI

英语

NI期刊 ; NI论文

第一 ; 通讯

National Key R&D Program of China[2018YFA0902600] ; National Natural Science Foundation of China[21535001][81730051][21761142006] ; Shenzhen Bay Laboratory[SZBL2019062801004] ; Guangdong Province[2019ZT08Y191] ; C i ty o f Shenzhen[KQTD20190929172743294] ; Department of Education of Guangdong Province[2019KQNCX130]

Chemistry

Chemistry, Analytical

WOS:000589246100072

AMER CHEMICAL SOC

20204709505497

Biomolecules ; Centrifugation ; Microfluidics

Biological Materials and Tissue Engineering:461.2 ; Biology:461.9 ; Microfluidics:632.5.1 ; Biochemistry:801.2 ; Chemical Operations:802.3 ; Organic Compounds:804.1

CHEMISTRY

2-s2.0-85096121594

Scopus

1.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen, Guangdong,518055,China
2.Shenzhen Bay Laboratory,Shenzhen, Guangdong,518055,China

Chen，Yong,Mei，Yixin,Zhao，Xiaohui,et al. Reagents-loaded, automated assay that integrates recombinase-aided amplification and CaS12a nucleic acid detection for a point-of-care test[J]. ANALYTICAL CHEMISTRY,2020,92(21):14846-14852.

Chen，Yong,Mei，Yixin,Zhao，Xiaohui,&Jiang，Xingyu.(2020).Reagents-loaded, automated assay that integrates recombinase-aided amplification and CaS12a nucleic acid detection for a point-of-care test.ANALYTICAL CHEMISTRY,92(21),14846-14852.

Chen，Yong,et al."Reagents-loaded, automated assay that integrates recombinase-aided amplification and CaS12a nucleic acid detection for a point-of-care test".ANALYTICAL CHEMISTRY 92.21(2020):14846-14852.