Genome-centric microbiome analysis reveals solid retention time (SRT)-shaped species interactions and niche differentiation in food waste and sludge co-digesters

Wang，Chunxiao1; Wang，Yubo1; Wang，Yulin1; Cheung，Kin kuen2; Ju，Feng3; Xia，Yu4; Zhang，Tong1

2020-08-15

10.1016/j.watres.2020.115858

WATER RESEARCH   影响因子和分区

0043-1354

1879-2448

Co-digestion of food waste with sewage sludge is widely applied for waste stabilization and energy recovery around the world. However, the effect of solid retention time (SRT) on the microbial population dynamics, metabolism and interspecies interaction have not been fully elucidated. Here, the influence of SRTs (5-25 days) on the performance of the co-digestion system was investigated and state-of-the-art genome-centric metagenomic analysis was employed to uncover the dynamics and metabolic network of the key players underlying the well-functioned and poorly-functioned co-digestion microbial communities. The results of the microbial analyses indicated that SRT largely shaped microbial community structure by enriching the syntrophic specialist Syntrophomonas and CO/H ( formate)-using methanogen Methanocorpusculum in the well-functioned co-digester operated at SRT of 25 days, while selecting acid-tolerant populations Lactobacillus at SRT of 5 days. The metagenome assembled genomes (MAGs) of key players, such as Syntrophomonadaceae, Methanocorpusculum, and Mesotoga, were retrieved, additionally, the syntrophic acetate oxidation plus hydrogenotrophic methanogenesis (SAO-HM) were proposed as the dominant pathway for methane production. The metabolic interaction in the co-digestion microbial consortia was profiled by assigning MAGs into functional guilds. Functional redundancy was found in the bacterial groups in hydrolysis step, and the members in these groups reduced the direct competition by niche differentiation.

Co-digester Metabolic interaction Metagenome assembled genomes Microbial communities Solid retention time

SCI ; EI

英语

NI期刊 ; NI论文

其他

Themed-Based ResearchScheme (TRS)[T21-711/16-R] ; Drainage Service Department (DSD) of Hong Kong SAR, China[DEMP/2015/06]

Engineering ; Environmental Sciences & Ecology ; Water Resources

Engineering, Environmental ; Environmental Sciences ; Water Resources

WOS:000541434400003

PERGAMON-ELSEVIER SCIENCE LTD

20202308786937

Genes ; Sludge digestion ; Microorganisms ; Anaerobic digestion ; Metabolism

Sewage Treatment:452.2 ; Biological Materials and Tissue Engineering:461.2 ; Biology:461.9

ENVIRONMENT/ECOLOGY

2-s2.0-85085740755

Scopus

1.Environmental Microbiome Engineering and Biotechnology Laboratory,Center for Environmental Engineering Research,Department of Civil Engineering,The University of Hong Kong,Hong Kong SAR,Pokfulam Road,Hong Kong
2.Drainage Services Department,Hong Kong SAR,Hong Kong
3.Environmental Microbiome and Biotechnology Laboratory (EMBLab),School of Engineering,Westlake University,Hangzhou,18 Shilongshan Road,310024,China
4.State Environmental Protection Key Laboratory of Integrated Surface Water- Groundwater Pollution Control,School of Environmental Science and Engineering,Southern University of Science and Technology,Shenzhen,China

Wang，Chunxiao,Wang，Yubo,Wang，Yulin,et al. Genome-centric microbiome analysis reveals solid retention time (SRT)-shaped species interactions and niche differentiation in food waste and sludge co-digesters[J]. WATER RESEARCH,2020,181.

Wang，Chunxiao.,Wang，Yubo.,Wang，Yulin.,Cheung，Kin kuen.,Ju，Feng.,...&Zhang，Tong.(2020).Genome-centric microbiome analysis reveals solid retention time (SRT)-shaped species interactions and niche differentiation in food waste and sludge co-digesters.WATER RESEARCH,181.

Wang，Chunxiao,et al."Genome-centric microbiome analysis reveals solid retention time (SRT)-shaped species interactions and niche differentiation in food waste and sludge co-digesters".WATER RESEARCH 181(2020).