Detection of Circulating Tumor Cells by Fluorescence Microspheres-Mediated Amplification

Yin，Jiaxiang1,3,4; Deng，Jinqi3; Wang，Le2; Du，Chang1,4,5; Zhang，Wei3; Jiang，Xingyu2,3

2020

10.1021/acs.analchem.9b05844

ANALYTICAL CHEMISTRY   影响因子和分区

0003-2700

1520-6882

Here we describe a fluorescent microspheres-based separation and analysis that enables the isolation of circulating tumor cells (CTCs) from whole blood of patients with metastatic cancer and the identification of isolated CTCs in situ without immunostaining. This approach uses antibody-functionalized fluorescent polystyrene (PS) microspheres that can selectively bind to CTCs. The binding of CTCs and fluorescent PS microspheres leads to the formation of complexes of CTCs and fluorescent PS microspheres, thereby the CTCs are size-amplified and labeled simultaneously. A pyramidal microcavity array (PMCA) is fabricated using microfabrication technology to create a precise microfilter structure with a high aspect ratio. The PMCA filter device can effectively isolate microspheres-labeled CTCs, while allow hematologic cells to deform and pass through. Using this approach, CTCs are isolated and identified in 15 of 18 patients with metastatic colorectal cancer. This approach will open new possibilities for CTCs isolation and identification and can serve a versatile platform to facilitate CTCs analysis in diverse biomedical applications.

SCI ; EI

英语

NI期刊 ; NI论文

其他

National Natural Science Foundation of China[21535001][81730051][21761142006] ; National Key R&D Program of China[2018YFA0902600][2017YFA0205901] ; Open Project of Key Laboratory of Biomedical Engineering of Guangdong Province[KLBEMGD201901] ; Chinese Academy of Sc iences[QYZDJ-SSW-SLH039][121D11KYSB20170026][XDA16020902]

Chemistry

Chemistry, Analytical

WOS:000537144800025

AMER CHEMICAL SOC

20201908642259

Microfiltration ; Fluorescence ; Tumors ; Aspect ratio ; Medical applications ; Diseases

Biological Materials and Tissue Engineering:461.2 ; Light/Optics:741.1 ; Chemical Operations:802.3

CHEMISTRY

2-s2.0-85084296937

Scopus

1.Department of Biomedical Engineering,School of Materials Science and Engineering,South China University of Technology,Guangzhou,510641,China
2.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,No. 1088 Xueyuan Rd, Nanshan District,518055,China
3.Beijing Engineering Research Center for BioNanotechnology and CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety,CAS Center for Excellence in Nanoscience,National Center for NanoScience and Technology,Beijing,No. 11 Zhongguancun Beiyitiao,100190,China
4.National Engineering Research Center for Tissue Restoration and Reconstruction,South China University of Technology,Guangzhou,510006,China
5.Key Laboratory of Biomedical Materials Science and Engineering,Ministry of Education,Guangzhou,510006,China

Yin，Jiaxiang,Deng，Jinqi,Wang，Le,et al. Detection of Circulating Tumor Cells by Fluorescence Microspheres-Mediated Amplification[J]. ANALYTICAL CHEMISTRY,2020,92(10):6968-6976.

Yin，Jiaxiang,Deng，Jinqi,Wang，Le,Du，Chang,Zhang，Wei,&Jiang，Xingyu.(2020).Detection of Circulating Tumor Cells by Fluorescence Microspheres-Mediated Amplification.ANALYTICAL CHEMISTRY,92(10),6968-6976.

Yin，Jiaxiang,et al."Detection of Circulating Tumor Cells by Fluorescence Microspheres-Mediated Amplification".ANALYTICAL CHEMISTRY 92.10(2020):6968-6976.