Nanoliposome-encapsulated caged-GABA for modulating neural electrophysiological activity with simultaneous detection by microelectrode arrays

Xie, Jingyu1,2; Song, Yilin1,2; Dai, Yuchuan1,2; Li, Ziyue1,2; Gao, Fei1,2; Li, Xuanyu2,3; Xiao, Guihua1,2; Zhang, Yu1,2; Wang, Hao1,2; Lu, Zeying1,2; Jiang, Xingyu3,4; Zheng, Wenfu3; Cai, Xinxia1,2

2020-05-11

10.1007/s12274-020-2802-0

Nano Research   影响因子和分区

1998-0124

1998-0000

Effective and precise neural modulation with real-time detection in the brain is of great importance and represents a significant challenge. Nanoliposome-encapsulated light-sensitive compounds have excellent characteristics such as high temporal and spatial resolution, delayed drug clearance, and restricted drug biodistribution for neural modulation. In this study, we developed a nanoliposome-based delivery system for ruthenium-based caged GABA compounds (Nanolipo-Ru) to modulate neural activity and allow for real-time monitoring using the microelectrode arrays (MEAs). The Nanolipo-Ru nanoparticles had an average size of 134.10 +/- 4.30 nm and exhibited excellent stability for seven weeks. For the in vivo experiment in the rat, release of GABA by Nanolipo-Ru under blue light illumination resulted in an average firing rate reduction in interneurons and pyramidal neurons in the same brain region of 79.4% and 81.6%, respectively. Simultaneously, the average power of local field potentials in the 0-15 Hz range degraded from 4.34 to 0.85 mW. In addition, the Nanolipo-Ru nanoparticles have the potential to provide more flexible timing of modulation than unencapsulated RuBi-GABA in the experiments. These results indicated that Nanolipo-Ru could be an effective platform for regulating neuronal electrophysiology. Furthermore, nanoliposomes with appropriate modifications would render promising utilities for targeting of specific types of neurons in the future.

neural modulation nanoliposome microelectrode array light-sensitive drug

SCI ; EI

英语

其他

National Key Research and Development Program of nano science and technology of China[2017YFA0205902] ; National Natural Science Foundation of China[61527815][61960206012][61975206][61775216][61971400][61973292][61771452] ; Key Research Programs of Frontier Sciences, CAS[QYZDJ-SSW-SYS015][XDA16020902]

Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics

Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied

WOS:000531780700007

TSINGHUA UNIV PRESS

20202008654115

Nanoparticles ; Ruthenium compounds ; Brain ; Targeted drug delivery ; Modulation ; Controlled drug delivery ; Photosensitivity ; Neurons ; Microelectrodes

Biomedical Engineering:461.1 ; Biology:461.9 ; Light/Optics:741.1 ; Nanotechnology:761 ; Solid State Physics:933

Web of Science

1.Chinese Acad Sci, Inst Elect, State Key Lab Transducer Technol, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Natl Ctr NanoSci & Technol, Beijing Engn Res Ctr BioNanotechnol, CAS Key Lab Biol Effects Nanomat & Nanosafety, CAS Ctr Excellence Nanosci, Beijing 100190, Peoples R China
4.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Peoples R China

Xie, Jingyu,Song, Yilin,Dai, Yuchuan,et al. Nanoliposome-encapsulated caged-GABA for modulating neural electrophysiological activity with simultaneous detection by microelectrode arrays[J]. Nano Research,2020,13(6):1756-1763.

Xie, Jingyu.,Song, Yilin.,Dai, Yuchuan.,Li, Ziyue.,Gao, Fei.,...&Cai, Xinxia.(2020).Nanoliposome-encapsulated caged-GABA for modulating neural electrophysiological activity with simultaneous detection by microelectrode arrays.Nano Research,13(6),1756-1763.

Xie, Jingyu,et al."Nanoliposome-encapsulated caged-GABA for modulating neural electrophysiological activity with simultaneous detection by microelectrode arrays".Nano Research 13.6(2020):1756-1763.